Ko te tikanga o te mahi o whākōkī

Whākōkī (whākōkī) - he tiketike rāpoi taimaha matū o te natura pūmua, e mahi i roto i te rauropi he wāhi o kaiwhakakökï koiora.

Ko te tikanga o te mahi o whākōkī

Elucidation o nga tikanga whakakaupapa i te mahi catalytic o whākōkī, ko tetahi o nga raruraru taketake me ngā raruraru o nāianei enzymology kore anake, engari mataora hou, me te koiora rāpoi.

Roa i mua ka wātea parakore nga whākōkī, a i elucidated ratou natura, kua riro tuipau e tino hira mō te tukanga enzymatic kua he hononga o whākōkī ki te tïpako. Ngana ki te kitea he pūhui matatini o te pūmua whākōkī ki te tïpako mo te wa roa kihai i arahi ki te angitu, rite tenei matatini he labile, reira rawa tere decomposes. hanga Mā te tikanga hihinga kua reira taea ki te tautuhi i te complexes paru-tïpako ki catalase, peroxidase, waipiro dehydrogenase, whākōkī flavinzavisimyh.

Ko te tikanga o te tātari X-ray kua whakaaetia ki te tiki i te rota o te mōhiohio nui i runga i te hanganga me te huarahi catalytic o mahi o whākōkī. I whakamahia tēnei aratuka ki te whakatū i te analogues tïpako hononga ki whākōkī lysozyme me chymotrypsin.

Ētahi taunakitanga tika mo te oraraa o complexes paru-tïpako whakahaere ki te tiki ki te wahi tetahi o nga wāhanga huringa catalytic o te whākōkī e herea ki te tïpako i te here kotuari. He tauira ko te tauhohenga o te tauhohenga wai o tāpia n-nitrophenyl catalyzed e chymotrypsin. A, no te konatunatua ki te Etera paru chymotrypsin acetylated te i te rōpū hydroxyl o te toenga serine urupare. Ko te tere tenei wā, engari atsetilhimotripsina paparetanga ki te hanganga o tāpia me free chymotrypsin ko nui pōturi. Na reira atsetilhimotripsin piki, kua ngāwari i kitea i roto i te aroaro o te n-nitrophenylacetate.

Ko te aroaro o te tïpako whākōkī i roto i te hanganga e taea "hao" e whakawhiti EU matatini eui i roto i te puka hohekore, hei tauira, i te maimoatanga ki te matatini paru-tïpako o konutai borohydride he pānga whakaiti kaha. kitea he matatini taua i roto i te puka o te pārōnaki kotuari pūmau i roto i te aldolase whākōkī. I kitea te reira e pähekoheko te tïpako ki te rōpū e-amino rāpoi ngota o te lysine.

tauhohe te tïpako ki te paru i roto i te wahi ngā huaina nei ko te pokapū kaha o te whākōkī te rohe hohe ranei.

I raro i te pokapū kaha, matua ranei, mahino e wahi paru muingota o te pūmua hono nei ki te tïpako (me cofactors) me ai te āhuatanga enzymatic o te rāpoi ngota. whakatau te pae mātātoa te pū, me te mahi catalytic o te paru me te kia waiho he tetahi tohu o te hanganga uaua urutau mō te huarahi tata, me te taunekeneke ki te rāpoi ngota tïpako, ranei ona wahi whai wāhi tika i roto i te tauhohenga.

I roto i E kitea ngā te rōpū mahi i roto i te "kaha catalytically" wahi o te pūmua whākōkī, me te wahi hanga whakarato i te marena motuhake (te here o te tïpako ki te whākōkī) - te pera-ka karanga titi, ranei "punga" (ranei adsorption o te pae kaha whākōkī).

whakamārama te tikanga o te mahi o whākōkī te ariā o te whārite Michaelis-Menten. E ai ki tenei ariā, e te tukanga wahi i roto i te wha wāhanga.

Ko te tikanga o te mahi o whākōkī: e ahau Wāhanga

I waenganui i te tïpako (C) me te paru (E) whakatika te hononga - i hanga EU paru-tïpako matatini, e toaati ai te wāhanga i te kotuari, ngota, te wai, me te tahi atu here.

Ko te tikanga o te mahi o whākōkī: IІ atamira

whakahohe te tïpako te whai wāhi ki te paru piri te ka riro e wātea ana mo te tauhohe tēnā o te catalysis EU.

Ko te tikanga o te mahi o whākōkī: atamira IІI

tukua EU catalysis. tautoko ana tēnei ariā e rangahau whakamātautau.

I te pae hopea, te āhuatanga IV atamira e te tuku o te rāpoi ngota paru E ko F. taea te whakaatu i te raupapa o panoni o ngā hua rite te tauhohe: E + S - EU EU - * - E + P.

Ko te pū o te mahi pūmua whākōkī

mahi ia pūmua whākōkī i runga i te tïpako motuhake ranei rōpū o ngā matū, e te hunga rite i roto i te hanganga. Pū o te mahi enzymatic tika ki te ōrite o te whirihoranga pae mātātoa, me te tïpako. I roto i te pāhekoheko o te paru-tïpako i hanga matatini.